Fluorescence In Situ Hybridization (FISH)
What is a FISH Test?
Fluorescence In Situ Hybridization (FISH) is a procedure that essentially creates a map of the genetic material in human cells, allowing cytogeneticists to locate specific DNA sequences on a chromosome. The process can give useful insight in the understanding of certain genetic mutations and chromosomal abnormalities.
How Does FISH Testing Work?
Using molecular biology techniques, FISH testing detects the presence (or absence of) specific regions of the genome. Additionally, the Fluorescence In Situ Hybridization procedure can be used to enumerate certain regions of the genome, depending on the circumstances.
Detection is accomplished by introducing fluorescently labeled pieces of DNA that are referred to as “probes”. Probes can be designed for any gene, size, or sequence. The process allows the cellular reproduction cycle to be mapped and analyzed.
When Is FISH Testing Used?
Cytogeneticists use FISH to identify genetic abnormalities related to cancer, as well as other diseases and conditions. In addition to being used for the purpose of detection, FISH can provide supplementary information that can be used to predict a patient’s response to certain treatment types.
Unlike most other techniques used to study chromosomes, FISH does not require actively dividing cells. This adds flexibility and increases the ability to identify and characterize cytogenetic abnormalities. Contact CMG to learn more about how we use FISH.
FISH TESTS OFFERED AT CENTER FOR MEDICAL GENETICS
- Acute Myeloid Leukemia - PML/RARA
- Chronic Myeloid Leukemia - BCR/ABL
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